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KMID : 0613820200300040321
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Journal of Life Science
2020 Volume.30 No. 4 p.321 ~ p.330
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Exposure to Copper (II) Chloride Induces Behavioral and Endocrine Changes in Zebrafish
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Sung Ji-Won
Lee Jeong-Won
Lee Seung-Heon
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Abstract
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The aim of this study was to investigate the effect of copper (II) chloride (CuCl2) on zebrafish. Zebrafish were exposed to various CuCl2 concentrations and subjected to different exposure times to determine the median lethal concentration (LC50) values. To evaluate stress responses, we measured whole-body cortisol levels and behavioral parameters using the open field test (OFT) or the novel tank test (NTT). The zebrafish were exposed to CuCl2 solution at concentrations of 1.5?150 μg/l or a vehicle for 1 hr before behavioral tests or sample collection for whole-body cortisol. The LC50 values were 30.3, 25.3, and 14.8 μg/l at 24, 48, and 96 hr, respectively. The NTT showed that mobility, velocity, and distance covered were significantly lower in zebrafish exposed to CuCl2 than in the control group (p<0.05), while the turn angle was significantly higher in zebrafish exposed to a CuCl2 concentration of 150 μg/l than in the control group (p<0.05). The OFT also showed that mobility, velocity, and distance covered were significantly lower and the turn angle and meandering were significantly higher in zebrafish exposed to all concentrations of CuCl2 than in the control group (p<0.05). The whole-body cortisol levels were significantly higher in zebrafish exposed to CuCl2 than in the control group (p<0.05). These results suggest that exposure to lethal CuCl2 concentrations induces an intense toxic and stress response in zebrafish, causing behavioral changes and increasing whole-body cortisol levels.
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KEYWORD
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Behavioral test, CuCl2, heavy metals, Toxicity Zebrafish
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